High-throughput screening of cell-free riboswitches by fluorescence-activated droplet sorting.

ABSTRACT: Cell-free systems that display complex functions without using living cells are emerging as new platforms to test our understanding of biological systems as well as for practical applications such as biosensors and biomanufacturing. Those that use cell-free protein synthesis (CFPS) systems to enable genetically programmed protein synthesis have relied on genetic regulatory components found or engineered in living cells. However, biological constraints such as cell permeability, metabolic stability, and toxicity of signaling molecules prevent development of cell-free devices using living cells even if cell-free systems are not subject to such constraints. Efforts to engineer regulatory components directly in CFPS systems thus far have been based on low-throughput experimental approaches, li

SUBMITTER: Tabuchi T 

PROVIDER: S-EPMC8989549 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature