High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks.

ABSTRACT: The biophysical properties of existing optogenetic tools constrain the scale, speed, and fidelity of precise optogenetic control. Here, we use structure-guided mutagenesis to engineer opsins that exhibit very high potency while retaining fast kinetics. These new opsins enable large-scale, temporally and spatially precise control of population neural activity. We extensively benchmark these new opsins against existing optogenetic tools and provide a detailed biophysical characterization of a diverse family of opsins under two-photon illumination. This establishes a resource for matching the optimal opsin to the goals and constraints of patterned optogenetics experiments. Finally, by combining these new opsins with optimized procedures for holographic photostimulation, we demonstrate the sim

SUBMITTER: Sridharan S 

PROVIDER: S-EPMC8989680 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature