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Functional and structural deficiencies of Gemin5 variants associated with neurological disorders.

ABSTRACT: Dysfunction of RNA-binding proteins is often linked to a wide range of human disease, particularly with neurological conditions. Gemin5 is a member of the survival of the motor neurons (SMN) complex, a ribosome-binding protein and a translation reprogramming factor. Recently, pathogenic mutations in Gemin5 have been reported, but the functional consequences of these variants remain elusive. Here, we report functional and structural deficiencies associated with compound heterozygosity variants within the Gemin5 gene found in patients with neurodevelopmental disorders. These clinical variants are located in key domains of Gemin5, the tetratricopeptide repeat (TPR)-like dimerization module and the noncanonical RNA-binding site 1 (RBS1). We show that the TPR-like variants disrupt protein dimerization, whereas the RBS1 variant confers protein instability. All mutants are defective in the interaction with protein networks involved in translation and RNA-driven pathways. Importantly, the TPR-like variants fail to associate with native ribosomes, hampering its involvement in translation control and establishing a functional difference with the wild-type protein. Our study provides insights into the molecular basis of disease associated with malfunction of the Gemin5 protein.

SUBMITTER: Francisco-Velilla R

PROVIDER: S-EPMC8989681 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Functional and structural deficiencies of Gemin5 variants associated with neurological disorders.

Francisco-Velilla Rosario R Embarc-Buh Azman A Del Caño-Ochoa Francisco F Abellan Salvador S Vilar Marçal M Alvarez Sara S Fernandez-Jaen Alberto A Kour Sukhleen S Rajan Deepa S DS Pandey Udai Bhan UB Ramón-Maiques Santiago S Martinez-Salas Encarnacion E

Life science alliance 20220407 7

Dysfunction of RNA-binding proteins is often linked to a wide range of human disease, particularly with neurological conditions. Gemin5 is a member of the survival of the motor neurons (SMN) complex, a ribosome-binding protein and a translation reprogramming factor. Recently, pathogenic mutations in <i>Gemin5</i> have been reported, but the functional consequences of these variants remain elusive. Here, we report functional and structural deficiencies associated with compound heterozygosity vari ...[more]

PMID: 35393353

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Functional and structural deficiencies of Gemin5 variants associated with neurological disorders.

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Functional and structural deficiencies of Gemin5 variants associated with neurological disorders.

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