Circ_0075825 promotes gastric cancer progression via adsorbing miR-432-5p to modulate SOX9.

ABSTRACT:

Methods

Circ_0075825 expression in adjacent tissues and GC tissues was evaluated by bioinformatics method and quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). How circ_0075825 regulated GC cell growth, migration, invasion, and apoptosis were investigated by cell counting kit-8 assay, transwell assay and flow cytometry. The targeted interplays among circ_0075825, and miR-432-5p and Sex-Determining Region Y-related high-mobility group box 9 (SOX9) were explored by bioinformatics analysis and luciferase reporter gene assay. The regulatory effects of circ_0075825 and miR-432-5p on SOX9 protein expression were probed by western blot.

Results

Circ_0075825 expression was raised in GC tissues and cell lines. Circ_0075825 overexpression promoted the proliferati

SUBMITTER: Li H 

PROVIDER: S-EPMC8989707 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature