Hydroxychloroquine Alleviates EAU by Inhibiting Uveitogenic T Cells and Ameliorating Retinal Vascular Endothelial Cells Dysfunction.

ABSTRACT:

Purpose

Inflammation triggers the activation of CD4⁺T cells and the breakdown of blood-retinal barrier, thus contributing to the pathology of experimental autoimmune uveitis (EAU). We explored the anti-inflammatory effect of hydroxychloroquine (HCQ) on EAU and the potential mechanisms active in T cells and retinal vascular endothelial cells (RVECs).

Methods

C57BL/6J mice were immunized with interphotoreceptor retinoid binding protein 1-20 (IRBP_1-20) to induce EAU and then treated with the vehicle or HCQ (100 mg/kg/day). On day 7, 14, 21, 30 and 60 after immunization, clinical scores were evaluated. On day 14, histopathological scores were assessed, and retinas, spleens, and lymph nodes were collected for quantitative polymerase chain reaction or flow cy

SUBMITTER: Hu Y 

PROVIDER: S-EPMC8989724 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature