Monoclonal Antibodies as Probes to Study Ligand-Induced Conformations of Troponin Subunits.

ABSTRACT: Striated muscle contraction and relaxation is regulated by Ca²⁺ at the myofilament level via conformational modulations of the troponin complex. To understand the structure-function relationship of troponin in normal muscle and in myopathies, it is necessary to study the functional effects of troponin isoforms and mutations at the level of allosteric conformations of troponin subunits. Traditional methodologies assessing such conformational studies are laborious and require significant amounts of purified protein, while many current methodologies require non-physiological conditions or labeling of the protein, which may affect their physiological conformation and function. To address these issues, we developed a novel approach using site-specific monoclonal antibodies (mA

SUBMITTER: Rasmussen M 

PROVIDER: S-EPMC8990283 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature