Structure, Function and Protein Engineering of Cereal-Type Inhibitors Acting on Amylolytic Enzymes.

ABSTRACT: Numerous plants, including cereals, contain seed proteins able to inhibit amylolytic enzymes. Some of these inhibitors, the CM-proteins (soluble in chloroform:methanol mixtures)-also referred to as cereal-type inhibitors (CTIs)-are the topic of this review. CM-proteins were first reported 75 years ago. They are small sulfur-rich proteins of the prolamine superfamily embracing bifunctional α-amylase/trypsin inhibitors (ATIs), α-amylase inhibitors (AIs), limit dextrinase inhibitors (LDIs), and serine protease inhibitors. Phylogenetically CM-proteins are predicted across poaceae genomes and many isoforms are identified in seed proteomes. Their allergenicity and hence adverse effect on humans were recognized early on, as were their roles in plant defense. Generally, CTIs target e

SUBMITTER: Moller MS 

PROVIDER: S-EPMC8990303 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature