MGP promotes CD8⁺ T cell exhaustion by activating the NF-κB pathway leading to liver metastasis of colorectal cancer.

ABSTRACT: Matrix Gla protein (MGP) was originally reported as a physiological suppressor of ectopia calcification and has also been reported to be associated with cancer. However, the relation between the biological functions of MGP and the immune response in colorectal cancer (CRC) remains unclear. Here, we investigated the regulatory role of MGP in the immune microenvironment of CRC. MGP expression in CRC samples was assessed by single-cell RNA sequencing and the Gene Expression Omnibus (GEO) database, and confirmed by quantitative real-time Polymerase Chain Reaction (qRT-PCR) and immunohistochemistry analysis of human CRC samples. The effect of MGP on proliferation and invasion of CRC cells was evaluated by in vitro assays involving MGP knockdown and overexpression. Luciferase reporter ass

SUBMITTER: Rong D 

PROVIDER: S-EPMC8990480 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature