Dissecting Electronic-Structural Transitions in the Nitrogenase MoFe Protein P-Cluster during Reduction.

ABSTRACT: The [8Fe-7S] P-cluster of nitrogenase MoFe protein mediates electron transfer from nitrogenase Fe protein during the catalytic production of ammonia. The P-cluster transitions between three oxidation states, P^N, P⁺, P²⁺ of which P^N↔P⁺ is critical to electron exchange in the nitrogenase complex during turnover. To dissect the steps in formation of P⁺ during electron transfer, photochemical reduction of MoFe protein at 231-263 K was used to trap formation of P⁺ intermediates for analysis by EPR. In complexes with CdS nanocrystals, illumination of MoFe protein led to reduction of the P-cluster P²⁺ that was coincident with formation of three distinct EPR signals: S = 1/2 axial and rhombic signals, an

SUBMITTER: Chica B 

PROVIDER: S-EPMC8991001 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature