Dataset Information

Tryptophan Supplementation Increases the Production of Microbial-Derived AhR Agonists in an In Vitro Simulator of Intestinal Microbial Ecosystem.

ABSTRACT: The aryl hydrocarbon receptor (AhR) plays an important role in intestinal homeostasis, and some microbial metabolites of tryptophan are known AhR agonists. In this study, we assessed the impact of tryptophan supplementation on the formation of tryptophan metabolites, AhR activation, and microbiota composition in the simulator of the human intestinal microbial ecosystem (SHIME). AhR activation, microbial composition, and tryptophan metabolites were compared during high tryptophan supplementation (4 g/L tryptophan), control, and wash-out periods. During tryptophan supplementation, the concentration of several tryptophan metabolites was increased compared to the control and wash-out period, but AhR activation by fermenter supernatant was significantly decreased. This was due to the higher levels of tryptophan, which was found to be an antagonist of AhR signaling. Tryptophan supplementation induced most microbial changes in the transverse colon including increased relative abundance of lactobacillus. We conclude that tryptophan supplementation leads to increased formation of AhR agonists in the colon.

SUBMITTER: Koper JE

PROVIDER: S-EPMC8991005 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Tryptophan Supplementation Increases the Production of Microbial-Derived AhR Agonists in an <i>In Vitro</i> Simulator of Intestinal Microbial Ecosystem.

Koper Jonna Eb JE Troise Antonio Dario AD Loonen Linda Mp LM Vitaglione Paola P Capuano Edoardo E Fogliano Vincenzo V Wells Jerry M JM

Journal of agricultural and food chemistry 20220328 13

The aryl hydrocarbon receptor (AhR) plays an important role in intestinal homeostasis, and some microbial metabolites of tryptophan are known AhR agonists. In this study, we assessed the impact of tryptophan supplementation on the formation of tryptophan metabolites, AhR activation, and microbiota composition in the simulator of the human intestinal microbial ecosystem (SHIME). AhR activation, microbial composition, and tryptophan metabolites were compared during high tryptophan supplementation ...[more]

PMID: 35344652

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Dataset Information

Tryptophan Supplementation Increases the Production of Microbial-Derived AhR Agonists in an In Vitro Simulator of Intestinal Microbial Ecosystem.

Publications

Tryptophan Supplementation Increases the Production of Microbial-Derived AhR Agonists in an <i>In Vitro</i> Simulator of Intestinal Microbial Ecosystem.

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