REDD1 gene knockout alleviates vascular smooth muscle cell remodeling in pulmonary hypertension.

ABSTRACT:

Objectives

Regulated in development and DNA damage responses 1 (REDD1) is an important transcription factor regulating mitochondria homeostasis, which is the important pathological alteration of pulmonary hypertension (PH). However, it is unclear whether REDD1 regulates the PASMCs mitochondria homeostasis by the similar mechanism in pulmonary arterial remodeling induced by hypoxia.

Methods

The global REDD1-knockout rats (REDD1-KO) on Sprague-Dawley background were used to generate a chronic hypoxia model of PH. Right ventricular hypertrophy and vascular remodeling were detected after exposure to hypoxia. Additionally, proliferation, apoptosis, migration, mitochondria homeostasis, and autophagy were performed in vivo and in vitro.

Results

The current rese

SUBMITTER: Fang X 

PROVIDER: S-EPMC8991113 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature