Antibody-conjugated silica-coated gold nanoparticles in targeted therapy of cervical cancer.

ABSTRACT: This study aimed to synthesize silica-coated gold (Au@SiO₂) nanoparticles coupled to antibodies against the scavenger receptor class B type I (SR-BI) and investigate their potential ability of visual tracking and treatment of cervical cancer. The fluorescein isothiocyanate (FITC)-labeled Au@SiO₂-SR-BI antibody was synthesized, followed by characterization determination. The expression and location of SR-BI protein in cervical cancer cells were respectively detected by western blot and immunofluorescence assays. The effects of nanoparticles on cancer cells were determined by adsorption assay and apoptosis detection, respectively. The effects of nanoparticles on tumor formation in nude mice were determined. The particle sizes of Au@SiO₂ ranged from 2-2.5 μm,

SUBMITTER: Yu H 

PROVIDER: S-EPMC8991142 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature