Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry.
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ABSTRACT:
SUBMITTER: Menzel L
PROVIDER: S-EPMC8991252 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
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Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry.
Menzel Lutz L Zschummel Maria M Rehm Armin A
STAR protocols 20220406 2
Blood endothelial cells (BECs) in lymph nodes are distinct stromal cells with a transcriptional profile allowing fast and specific adaptation to the functional requirements. Here, we describe a step-by-step protocol for the enzymatic digestion of lymph nodes, the enrichment of stromal cells, the sorting of BECs, and the processing of BEC-related data for modern analysis approaches as spectral flow cytometry and single-cell RNA sequencing (scRNA-seq). For complete details on the use and execution ...[more]
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