Protein purification strategies must consider downstream applications and individual biological characteristics.

ABSTRACT:

Background

Proteins are used as reagents in a broad range of scientific fields. The reliability and reproducibility of experimental data will largely depend on the quality of the (recombinant) proteins and, consequently, these should undergo thorough structural and functional controls. Depending on the downstream application and the biochemical characteristics of the protein, different sets of specific features will need to be checked.

Results

A number of examples, representative of recurrent issues and previously published strategies, has been reported that illustrate real cases of recombinant protein production in which careful strategy design at the start of the project combined with quality controls throughout the production process was imperative to obtain high-quality

SUBMITTER: Remans K 

PROVIDER: S-EPMC8991485 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature