Isolation, purification and characterization of the antibacterial, antihypertensive and antioxidative properties of the bioactive peptides in the purified and proteolyzed major storage protein of pigeon pea (Cajanus cajan) seeds.

ABSTRACT: Previous studies in pigeon pea showed health benefits but very few explored peptide bioactivities. This study examined antimicrobial, antioxidant, and antihypertensive activities of peptides in purified and proteolyzed major globulin fraction, cajanin, of pigeon pea (Cajanus cajan) seeds. In-silico analysis showed 41 antihypertensive and nine antioxidant peptides but zero antibacterial peptides from cajanin sequence. The Pepsin-Chymotrypsin-Trypsin (PCT) protein digest has no antibacterial activity against Escherichia coli, Candida albicans, and Staphylococcus aureus but has high % ACE inhibition (87.50%). Two HPLC fractions showed low IC₅₀ values (HPLC Fraction 1: 0.00535 mg/ml; HPLC Fraction 2: 0.00432 mg/ml), comparable to Captopril (0.00379 mg/ml)

SUBMITTER: Bravo RKD 

PROVIDER: S-EPMC8991556 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature