Development and characterization of a glycine biosensor system for fine-tuned metabolic regulation in Escherichia coli.

ABSTRACT:

Background

In vivo biosensors have a wide range of applications, ranging from the detection of metabolites to the regulation of metabolic networks, providing versatile tools for synthetic biology and metabolic engineering. However, in view of the vast array of metabolite molecules, the existing number and performance of biosensors is far from sufficient, limiting their potential applications in metabolic engineering. Therefore, we developed the synthetic glycine-ON and -OFF riboswitches for metabolic regulation and directed evolution of enzyme in Escherichia coli.

Results

The results showed that a synthetic glycine-OFF riboswitch (glyOFF6) and an increased-detection-range synthetic glycine-ON riboswitch (glyON14) were successfully screened from a library based on the Bacillu

SUBMITTER: Hong KQ 

PROVIDER: S-EPMC8991567 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature