SETDB1 interactions with PELP1 contributes to breast cancer endocrine therapy resistance.

ABSTRACT:

Background

Methyltransferase SETDB1 is highly expressed in breast cancer (BC), however, the mechanisms by which SETDB1 promotes BC progression to endocrine therapy resistance remains elusive. In this study, we examined the mechanisms by which SETDB1 contribute to BC endocrine therapy resistance.

Methods

We utilized therapy sensitive (MCF7 and ZR75), therapy resistant (MCF7-TamR, MCF7-FR, MCF7-PELP1cyto, MCF7-SETDB1) estrogen receptor alpha positive (ER⁺)BC models and conducted in vitro cell viability, colony formation, 3-dimensional cell growth assays to investigate the role of SETDB1 in endocrine resistance. RNA-seq of parental and SETDB1 knock down ER⁺ BC cells was used to identify unique pathways. SETDB1 interaction with PELP1 was identified by yeast

SUBMITTER: Liu Z 

PROVIDER: S-EPMC8991965 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature