Reductive site-selective atypical C,Z-type/N2-C2 cleavage allows C-terminal protein amidation.

ABSTRACT: Biomolecule environments can enhance chemistries with the potential to mediate and modulate self-modification (e.g., self-cleavage). While these enhanced modes are found in certain biomolecules (e.g., RNA ribozymes), it is more rare in proteins. Targeted proteolytic cleavage is vital to physiology, biotechnology, and even emerging therapy. Yet, purely chemically induced methods for the site-selective cleavage of proteins remain scarce. Here, as a proof of principle, we designed and tested a system intended to combine protein-enhanced chemistry with tag modification to enable synthetic reductive protein chemistries promoted by diboron. This reductively driven, single-electron chemistry now enables an operationally simple, site-selective cleavage protocol for proteins directed to readily acc

SUBMITTER: Mollner TA 

PROVIDER: S-EPMC8993120 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature