Saponin-based adjuvant-induced dendritic cell cross-presentation is dependent on PERK activation.

ABSTRACT: Saponin-based adjuvants (SBAs) are promising new adjuvants that stand out as they not only enforce CD4 + T cell-mediated immunity and antibody responses, but also induce an unprecedented level of antigen cross-presentation by dendritic cells (DC) and subsequent CD8 + T cell activation. We discovered that SBA's ability to boost cross-presentation depends on the induction of lipid bodies (LBs). Moreover, the MHCII^loCD11b^hi DC subset was identified to be most responsive to SBA-induced cross-presentation. The aim is to further unravel the mechanisms behind the induction of DC cross-presentation by SBAs. Here we show that SBAs specifically induce the PKR-like Endoplasmic Reticulum kinase (PERK) pathway and that SBA-induced DC cross-presentation is dependent on activation o

SUBMITTER: Huis In 't Veld LGM 

PROVIDER: S-EPMC8994093 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature