A map of neurofilament light chain species in brain and cerebrospinal fluid and alterations in Alzheimer's disease.

ABSTRACT: Neurofilament light is a well-established marker of both acute and chronic neuronal damage and is increased in multiple neurodegenerative diseases. However, the protein is not well characterized in brain tissue or body fluids, and it is unknown what neurofilament light species are detected by commercial assays and whether additional species exist. We developed an immunoprecipitation-mass spectrometry assay using custom antibodies targeting various neurofilament light domains, including antibodies targeting Coil 1A/1B of the rod domain (HJ30.13), Coil 2B of the rod domain (HJ30.4) and the tail region (HJ30.11). We utilized our assay to characterize neurofilament light in brain tissue and CSF of individuals with Alzheimer's disease dementia and healthy controls. We then validated a quantitat

SUBMITTER: Budelier MM 

PROVIDER: S-EPMC8994116 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature