AdpA, a developmental regulator, promotes ε-poly-L-lysine biosynthesis in Streptomyces albulus.

ABSTRACT:

Background

AdpA is a global regulator of morphological differentiation and secondary metabolism in Streptomyces, but the regulatory roles of the Streptomyces AdpA family on the biosynthesis of the natural product ε-poly-L-lysine (ε-PL) remain unidentified, and few studies have focused on increasing the production of ε-PL by manipulating transcription factors in Streptomyces.

Results

In this study, we revealed the regulatory roles of different AdpA homologs in ε-PL biosynthesis and morphological differentiation and effectively promoted ε-PL production and sporulation in Streptomyces albulus NK660 by heterologously expressing adpA from S. neyagawaensis NRRLB-3092 (adpA_Sn). First, we identified a novel AdpA homolog named AdpA_Sa in S. albulus NK660 and char

SUBMITTER: Huang R 

PROVIDER: S-EPMC8994273 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature