A methanolic extract of Zanthoxylum bungeanum modulates secondary metabolism regulator genes in Aspergillus flavus and shuts down aflatoxin production.

ABSTRACT: Aflatoxin B1 (AFB1) is a food-borne toxin produced by Aspergillus flavus and a few similar fungi. Natural anti-aflatoxigenic compounds are used as alternatives to chemical fungicides to prevent AFB1 accumulation. We found that a methanolic extract of the food additive Zanthoxylum bungeanum shuts down AFB1 production in A. flavus. A methanol sub-fraction (M20) showed the highest total phenolic/flavonoid content and the most potent antioxidant activity. Mass spectrometry analyses identified four flavonoids in M20: quercetin, epicatechin, kaempferol-3-O-rhamnoside, and hyperoside. The anti-aflatoxigenic potency of M20 (IC₅₀: 2-4 µg/mL) was significantly higher than its anti-proliferation potency (IC₅₀: 1800-1900 µg/mL). RNA-seq data indicated that M20 triggers significan

SUBMITTER: Abbas A 

PROVIDER: S-EPMC8994782 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature