Immobilization of catalase on functionalized magnetic nanoparticles: a statistical approach.

ABSTRACT: Magnetic nanoparticles (MNPs) Fe₃O₄, by virtue of easily modifiable surface, high surface-to-mass ratio and super-paramagnetic properties, are one of suitable candidates for the enzyme immobilization. Optimization of five important variables viz. concentration of 3-aminopropyl-tri-ethoxy-silane (APTES), glutaraldehyde (GA) and enzyme, time and temperature of loading was carried out using central composite type of experimental design without blocks giving 50 experiments including eight replicates at the central point. Characterization, stability and reusability studies were also carried out with optimized preparation. Results established the correlation between observed and response surface method (RSM) equation envisaged value (R ² 0.99, 0.97

SUBMITTER: Goyal P 

PROVIDER: S-EPMC8994807 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature