LINC00511 enhances LUAD malignancy by upregulating GCNT3 via miR-195-5p.

ABSTRACT:

Background

Accumulating evidence suggests that LINC00511 acts as an oncogenic long non-coding RNA (lncRNA) in various cancers, including lung adenocarcinoma (LUAD). Hence, we attempted to elucidate the potential role of LINC00511 in LUAD.

Methods

LINC00511, miR-195-5p, and GCNT3 expression in LUAD was detected by qRT-PCR. Changes in the proliferation, migration, and invasion of LUAD cells after abnormal regulation of LINC00511, miR-195-5p, or GCNT3 were detected by CCK-8, BrdU, wound healing, and transwell assays. Bax and Bcl-2 protein expression was measured by western blotting. Additionally, we identified the targeting effects of LINC00511, miR-195-5p, and GCNT3 using luciferase and RNA immunoprecipitation (RIP) assays.

Results

LINC00511 and GCNT3 were found to be u

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8994914 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature