Novel insight into the role of the vitamin D receptor in the development and function of the immune system.

ABSTRACT: Immune cells express the vitamin D receptor (VDR) and are therefore vitamin D targets. The Vdr protein can be readily measured in the kidney using antibodies to the Vdr and western blot. It is much more difficult to measure Vdr protein in the spleen because of the low level of VDR expression in resting immune cells. In order to more sensitively measure VDR expression, the Cre enzyme was inserted in the 3rd exon of the VDR gene and a reporter mouse that irreversibly expresses tdTomato was made. Mice that express one copy of the VDRCre gene were confirmed to be VDR +/- and mice that express two copies were confirmed to be VDR -/-. Initial characterization of the immune cells from the VDR +/-/VDR^tdTomato+ mice, compared to VDR+/+ wildtype (WT) littermates, showed no effect of being

SUBMITTER: Arora J 

PROVIDER: S-EPMC8995385 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature