Analyses of erythropoiesis from embryonic stem cell-CD34⁺ and cord blood-CD34⁺ cells reveal mechanisms for defective expansion and enucleation of embryomic stem cell-erythroid cells.

ABSTRACT: Red blood cells (RBCs) generated ex vivo have the potential to be used for transfusion. Human embryonic stem cells (ES) and induced pluripotent stem cells (iPS) possess unlimited self-renewal capacity and are the preferred cell sources to be used for ex vivo RBC generation. However, their applications are hindered by the facts that the expansion of ES/iPS-derived erythroid cells is limited and the enucleation of ES/iPS-derived erythroblasts is low compared to that derived from cord blood (CB) or peripheral blood (PB). To address this, we sought to investigate the underlying mechanisms by comparing the in vitro erythropoiesis profiles of CB CD34⁺ and ES CD34⁺ cells. We found that the limited expansion of ES CD34⁺ cell-derived erythroid cells was associated w

SUBMITTER: Wang S 

PROVIDER: S-EPMC8995447 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature