GPR43 Suppresses Intestinal Tumor Growth by Modification of the Mammalian Target of Rapamycin Complex 1 Activity in ApcMin/+ Mice.

ABSTRACT:

Objective

G protein-coupled receptor 43 (GPR43), a receptor for short-chain fatty acids, plays a role in suppressing tumor growth; however, the detailed underlying mechanism needs to be comprehensively elucidated. In this study, we investigated the role of GPR43 in inhibiting tumor growth using ApcMin/+, a murine model of intestinal tumors.

Materials and methods

Using GPR43-/- ApcMin/+ and GPR43+/- ApcMin/+ mice, the number of tumors was analyzed at the end of the experimental period. Immunohistochemistry, quantitative polymerase chain reaction, and Western blotting were performed to analyze cellular proliferation and proliferation-associated signal pathways.

Results

Our results revealed that GPR43 deficiency resulted in increased tumor numbers in ApcMin/+ mice. Ki67

SUBMITTER: Kong L 

PROVIDER: S-EPMC8995667 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature