Local euchromatin enrichment in lamina-associated domains anticipates their repositioning in the adipogenic lineage.

ABSTRACT:

Background

Interactions of chromatin with the nuclear lamina via lamina-associated domains (LADs) confer structural stability to the genome. The dynamics of positioning of LADs during differentiation, and how LADs impinge on developmental gene expression, remains, however, elusive.

Results

We examined changes in the association of lamin B1 with the genome in the first 72 h of differentiation of adipose stem cells into adipocytes. We demonstrate a repositioning of entire stand-alone LADs and of LAD edges as a prominent nuclear structural feature of early adipogenesis. Whereas adipogenic genes are released from LADs, LADs sequester downregulated or repressed genes irrelevant for the adipose lineage. However, LAD repositioning only partly concurs with gene expression changes. D

SUBMITTER: Madsen-Osterbye J 

PROVIDER: S-EPMC8996409 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature