M2-like macrophages transplantation protects against the doxorubicin-induced heart failure via mitochondrial transfer.

ABSTRACT:

Aims

The alternatively activated macrophages have shown a cardioprotective effect in heart failure. However, the effect of M2 adoptive transfer in non-ischemic heart failure is unknown. In this study, we evaluated the efficacy of M-CSF plus IL-4 induced M2-like macrophages transplantation in doxorubicin-induced cardiotoxicity.

Methods

Bone marrow mononuclear cells were polarized as CCR2⁺CD206⁺ M2-like macrophages by a combination of M-CSF plus IL-4 treatment. C57BL/6 mice received a single intraperitoneal injection of doxorubicin (15 mg/kg). The treatment group were treated with M2-like macrophages (1 × 10^6 cells per mouse; i.v.) once a week for 2 weeks. After 3 weeks, we examined the percentage of resident cells and cardiac function. Furthermore, we e

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8996664 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature