Chromosome-specific telomere lengths and the minimal functional telomere revealed by nanopore sequencing.

ABSTRACT: We developed a method to tag telomeres and measure telomere length by nanopore sequencing in the yeast S. cerevisiae Nanopore allows long-read sequencing through the telomere, through the subtelomere, and into unique chromosomal sequence, enabling assignment of telomere length to a specific chromosome end. We observed chromosome end-specific telomere lengths that were stable over 120 cell divisions. These stable chromosome-specific telomere lengths may be explained by slow clonal variation or may represent a new biological mechanism that maintains equilibrium unique to each chromosome end. We examined the role of RIF1 and TEL1 in telomere length regulation and found that TEL1 is epistatic to RIF1 at most telomeres, consistent with the literature. However,

SUBMITTER: Sholes SL 

PROVIDER: S-EPMC8997346 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature