Inflammatory Caspase Activity Mediates HMGB1 Release and Differentiation in Myoblasts Affected by Peripheral Arterial Disease.

ABSTRACT:

Introduction

We previously showed that caspase-1 and -11, which are activated by inflammasomes, mediate recovery from muscle ischemia in mice. We hypothesized that similar to murine models, inflammatory caspases modulate myogenicity and inflammation in ischemic muscle disease.

Methods

Caspase activity was measured in ischemic and perfused human myoblasts in response to the NLRP3 and AIM2 inflammasome agonists (nigericin and poly(dA:dT), respectively) with and without specific caspase-1 or pan-caspase inhibition. mRNA levels of myogenic markers and caspase-1 were assessed, and protein levels of caspases-1, -4, -5, and -3 were measured by Western blot.

Results

When compared to perfused cells, ischemic myoblasts demonstrated attenuated MyoD and myogenin and elevated casp

SUBMITTER: Ferrari R 

PROVIDER: S-EPMC8997414 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature