HepG2-Based Designer Cells with Heat-Inducible Enhanced Liver Functions.

ABSTRACT: Functional human hepatocytes have been a pivotal tool in pharmacological studies such as those investigating drug metabolism and hepatotoxicity. However, primary human hepatocytes are difficult to obtain in large quantities and may cause ethical problems, necessitating the development of a new cell source to replace human primary hepatocytes. We previously developed genetically modified murine hepatoma cell lines with inducible enhanced liver functions, in which eight liver-enriched transcription factor (LETF) genes were introduced into hepatoma cells as inducible transgene expression cassettes. Here, we establish a human hepatoma cell line with heat-inducible liver functions using HepG2 cells. The genetically modified hepatoma cells, designated HepG2/8F_HS, actively proliferated under nor

SUBMITTER: Kitano H 

PROVIDER: S-EPMC8997820 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature