A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea.

ABSTRACT: The winged helix superfamily comprises a large number of structurally related nucleic acid-binding proteins. While these proteins are often shown to bind dsDNA, few are known to bind ssDNA. Here, we report the identification and characterization of Sul7s, a novel winged-helix single-stranded DNA binding protein family highly conserved in Sulfolobaceae. Sul7s from Sulfolobus islandicus binds ssDNA with an affinity approximately 15-fold higher than that for dsDNA in vitro. It prefers binding oligo(dT)₃₀ over oligo(dC)₃₀ or a dG-rich 30-nt oligonucleotide, and barely binds oligo(dA)₃₀. Further, binding by Sul7s inhibits DNA strand annealing, but shows little effect on the melting temperature of DNA duplexes. The solution structure of Sul7s determ

SUBMITTER: Huang C 

PROVIDER: S-EPMC8998557 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature