Effect of Betaine and Arginine on Interaction of αB-Crystallin with Glycogen Phosphorylase b.

ABSTRACT: Protein-protein interactions (PPIs) play an important role in many biological processes in a living cell. Among them chaperone-client interactions are the most important. In this work PPIs of αB-crystallin and glycogen phosphorylase b (Phb) in the presence of betaine (Bet) and arginine (Arg) at 48 °C and ionic strength of 0.15 M were studied using methods of dynamic light scattering, differential scanning calorimetry, and analytical ultracentrifugation. It was shown that Bet enhanced, while Arg reduced both the stability of αB-crystallin and its adsorption capacity (AC0) to the target protein at the stage of aggregate growth. Thus, the anti-aggregation activity of αB-crystallin increased in the presence of Bet and decreased under the influence of Arg, which resulted in inhibition or accele

SUBMITTER: Eronina TB 

PROVIDER: S-EPMC8998655 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature