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Monitoring Stimulated Darkening from UV-C Light on Different Bean Genotypes by NMR Spectroscopy.

ABSTRACT: The use of UV-C cool white light on bean (Phaseolus vulgaris L.) seeds significantly increases the biochemical seed coat post-harvest darkening process, whilst preserving seed germination. The aim of this work consists in monitoring the effect caused by the incidence of UV-C light on different bean genotypes using NMR spectroscopy. The genotype samples named IAC Alvorada; TAA Dama; BRS Estilo and BRS Pérola from the Agronomic Institute (IAC; Campinas; SP; Brazil) were evaluated. The following two methodologies were used: a prolonged darkening, in which the grain is placed in a room at a controlled temperature (298 K) and humidity for 90 days, simulating the supermarket shelf; an accelerated darkening, where the grains are exposed to UV-C light (254 nm) for 96 h. The experiments were performed using the following innovative time-domain (TD) NMR approaches: the RK-ROSE pulse sequence; one- and two-dimensional high resolution (HR) NMR experiments (1H; 1H-1H COSY and 1H-13C HSQC); chemometrics tools, such as PLS-DA and heat plots. The results suggest that the observed darkening occurs on the tegument after prolonged (90 days) and accelerated (96 h) conditions. In addition, the results indicate that phenylalanine is the relevant metabolite within this context, being able to participate in the chemical reactions accounted for by the darkening processes. Additionally, it is possible to confirm that a UV-C lamp accelerates oxidative enzymatic reactions and that the NMR methods used were a trustworthy approach to monitor and understand the darkening in bean seeds at metabolite level.

SUBMITTER: Salvador MV

PROVIDER: S-EPMC9000581 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Monitoring Stimulated Darkening from UV-C Light on Different Bean Genotypes by NMR Spectroscopy.

Salvador Marília Vilela MV Kock Flávio Vinícius Crizóstomo FVC Santos Isabella Laporte IL Paulino Jean Fausto Carvalho JFC de Almeida Caléo Panhoca CP Dos Santos Garcia Rodrigo Henrique RH Benchimol-Reis Luciana Lasry LL Colnago Luiz Alberto LA Ferreira Antonio Gilberto AG

Molecules (Basel, Switzerland) 20220323 7

The use of UV-C cool white light on bean (<i>Phaseolus vulgaris</i> L.) seeds significantly increases the biochemical seed coat post-harvest darkening process, whilst preserving seed germination. The aim of this work consists in monitoring the effect caused by the incidence of UV-C light on different bean genotypes using NMR spectroscopy. The genotype samples named IAC Alvorada; TAA Dama; BRS Estilo and BRS Pérola from the Agronomic Institute (IAC; Campinas; SP; Brazil) were evaluated. The follo ...[more]

PMID: 35408459

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Project description:Genotype by environment interaction (GxE) complicates the process of selecting genotypes suitable for quantitative traits like seed yield in beans, hence slows down the development and release of varieties by breeding programs. GxE study on seed yield in beans enables identification of stable genotypes across sites and best site(s) for discriminating the tested genotypes in terms of seed yield. The purpose of this study was to evaluate the influence of the environment, genotype, and genotype by environment interaction on seed yield stability and adaptability of common bean landraces, lines, and improved varieties across three different agro-ecologies in Tanzania. The 99 common bean genotypes (Landraces, lines, and improved varieties) were planted following alpha lattice design in three replications each contained five blocks with 20 plots. Soil properties from the experimental sites, days to 75% flowering, Seed yield, 100 seed weight, number of seeds/pod, and number of pods/plant were recorded. Data on seed yield and its components were analyzed using Additive main effect and multiplicative interaction (AMMI), genotype main effects plus genotype × environment interaction (GGE), and yield stability index (YSI). The AMMI revealed very highly significant (P ≤ 0.001) effects of genotypes, environmental, and genotype × environment interaction on all the traits. AMMI analysis revealed that genotype main effects accounted for 39.3% of the total sum square of seed yield, whereas the environment and genotype × environmental interaction accounted for 31.4% and 26.8 % respectively. Genotype main effects largely influenced the variation in days to 75% flowering (55.5%), number of pods/plant (49.2%), number of seeds/pod (73.3%), and 100 seed weight (71.2%). Among soil properties recorded, available soil phosphorus, soil pH, soil exchangeable K, Ca, and Na had a strong positive association with common bean seed yield, while soil organic carbon and total nitrogen exhibited a strong negative association with seed yield. GGE revealed that E1 (TARI-Selian) was the most discriminative and representative site for common bean genotypes seed yield. Based on the yield stability index, the most stable and high seed yielding genotypes were ACC 714, Selian 14, Selian 9, Katuku, and Msolini. The identified high seed yielding and stable genotypes can be further tested in participatory variety selection involving farmers and later on released as varieties and can also be used for different breeding purposes in different agro-ecologies of Tanzania.

Dataset Information

Monitoring Stimulated Darkening from UV-C Light on Different Bean Genotypes by NMR Spectroscopy.

Publications

Monitoring Stimulated Darkening from UV-C Light on Different Bean Genotypes by NMR Spectroscopy.

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