Modular, robust, and extendible multicellular circuit design in yeast.

ABSTRACT: Division of labor between cells is ubiquitous in biology but the use of multicellular consortia for engineering applications is only beginning to be explored. A significant advantage of multicellular circuits is their potential to be modular with respect to composition but this claim has not yet been extensively tested using experiments and quantitative modeling. Here, we construct a library of 24 yeast strains capable of sending, receiving or responding to three molecular signals, characterize them experimentally and build quantitative models of their input-output relationships. We then compose these strains into two- and three-strain cascades as well as a four-strain bistable switch and show that experimentally measured consortia dynamics can be predicted from the models of the constitue

SUBMITTER: Carignano A 

PROVIDER: S-EPMC9000959 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature