A targeted capture approach to generating reference sequence databases for chloroplast gene regions.

ABSTRACT: Metabarcoding has improved the way we understand plants within our environment, from their ecology and conservation to invasive species management. The notion of identifying plant taxa within environmental samples relies on the ability to match unknown sequences to known reference libraries. Without comprehensive reference databases, species can go undetected or be incorrectly assigned, leading to false-positive and false-negative detections. To improve our ability to generate reference sequence databases, we developed a targeted capture approach using the OZBaits_CP V1.0 set, designed to capture chloroplast gene regions across the entirety of flowering plant diversity. We focused on generating a reference database for coastal temperate plant species given the lack of reference sequences f

SUBMITTER: Foster NR 

PROVIDER: S-EPMC9001157 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature