Adenosine inhibits TNFα-induced MMP-3 production in MH7A rheumatoid arthritis synoviocytes via A_2A receptor signaling.

ABSTRACT: Adenosine causes the anti-inflammatory effect of MTX; however, the contributions of synoviocyte adenosine receptors (AdoRs) are unknown, and matrix metalloproteinase 3 (MMP-3) is released by fibroblast-like synoviocytes in response to inflammatory signaling. To understand the mechanism of the clinical observation that the matrix proteinase-3 concentration of patients with rheumatoid arthritis treated successfully with methotrexate does not usually normalize, we investigated the effects of A_2A AdoR activation and inhibition on tumor necrosis factor-alpha (TNFα)-induced MMP-3 release by MH7A human rheumatoid synovial cells. MH7A cells constitutively expressed membrane-associated A_2A AdoRs, and HENECA enhanced intracellular cAMP. Stimulation with TNFα markedly enhanced r

SUBMITTER: Konishi H 

PROVIDER: S-EPMC9001689 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature