Multidimensional chromatin profiling of zebrafish pancreas to uncover and investigate disease-relevant enhancers.

ABSTRACT: The pancreas is a central organ for human diseases. Most alleles uncovered by genome-wide association studies of pancreatic dysfunction traits overlap with non-coding sequences of DNA. Many contain epigenetic marks of cis-regulatory elements active in pancreatic cells, suggesting that alterations in these sequences contribute to pancreatic diseases. Animal models greatly help to understand the role of non-coding alterations in disease. However, interspecies identification of equivalent cis-regulatory elements faces fundamental challenges, including lack of sequence conservation. Here we combine epigenetic assays with reporter assays in zebrafish and human pancreatic cells to identify interspecies functionally equivalent cis-regulatory elements, regardless of sequence conservation. Among ot

SUBMITTER: Bordeira-Carrico R 

PROVIDER: S-EPMC9001708 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature