Strand asymmetry influences mismatch resolution during a single-strand annealing.

ABSTRACT:

Background

Biases of DNA repair can shape the nucleotide landscape of genomes at evolutionary timescales. The molecular mechanisms of those biases are still poorly understood because it is difficult to isolate the contributions of DNA repair from those of DNA damage.

Results

Here, we develop a genome-wide assay whereby the same DNA lesion is repaired in different genomic contexts. We insert thousands of barcoded transposons carrying a reporter of DNA mismatch repair in the genome of mouse embryonic stem cells. Upon inducing a double-strand break between tandem repeats, a mismatch is generated if the break is repaired through single-strand annealing. The resolution of the mismatch showed a 60-80% bias in favor of the strand with the longest 3' flap. The location of the lesion

SUBMITTER: Pokusaeva VO 

PROVIDER: S-EPMC9001825 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature