The pre-induction temperature affects recombinant HuGM-CSF aggregation in thermoinducible Escherichia coli.

ABSTRACT: The overproduction of recombinant proteins in Escherichia coli leads to insoluble aggregates of proteins called inclusion bodies (IBs). IBs are considered dynamic entities that harbor high percentages of the recombinant protein, which can be found in different conformational states. The production conditions influence the properties of IBs and recombinant protein recovery and solubilization. The E. coli growth in thermoinduced systems is generally carried out at 30 °C and then recombinant protein production at 42 °C. Since the heat shock response in E. coli is triggered above 34 °C, the synthesis of heat shock proteins can modify the yields of the recombinant protein and the structural quality of IBs. The objective of this work was to evaluate the effect of different pre-induction temperat

SUBMITTER: Restrepo-Pineda S 

PROVIDER: S-EPMC9002048 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature