Use of Nanopore Sequencing to Characterise the Genomic Architecture of Mobile Genetic Elements Encoding bla _CTX-M-15 in Escherichia coli Causing Travellers' Diarrhoea.

ABSTRACT: Increasing levels of antimicrobial resistance (AMR) have been documented in Escherichia coli causing travellers' diarrhoea, particularly to the third-generation cephalosporins. Diarrhoeagenic E. coli (DEC) can act as a reservoir for the exchange of AMR genes between bacteria residing in the human gut, enabling them to survive and flourish through the selective pressures of antibiotic treatments. Using Oxford Nanopore Technology (ONT), we sequenced eight isolates of DEC from four patients' specimens who had all recently returned to the United Kingdome from Pakistan. Sequencing yielded two DEC harbouring bla _CTX-M-15 per patient, all with different sequence types (ST) and belonging to five different pathotypes. The study aimed to determine whether bla <

SUBMITTER: Bird MT 

PROVIDER: S-EPMC9002331 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature