DNA-Based Identification of Eurasian Vicia Species Using Chloroplast and Nuclear DNA Barcodes.

ABSTRACT: Many legume species of the Vicia L. genus (Fabaceae Lindl.) are key components of the Mediterranean diet and have an integral role in sustainable agriculture. Given the importance of the Vicia species for Eurasian culture, it is necessary to implement methodologies, such as DNA barcoding, that can enable the effective authentication and identification of species in the genus. In this study, we analysed the chloroplast trnL and rpoC1, as well as the nuclear ITS2 DNA barcoding regions, to identify 71 Vicia specimens of Eurasian descent. Both the trnL and ITS2 regions were highly effective in discriminating the analysed taxa, while the more conserved rpoC1 region could not identify all of the selected species due to high sequence conservation o

SUBMITTER: Bosmali I 

PROVIDER: S-EPMC9003045 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature