Fluorescent Aptasensor for Highly Specific Detection of ATP Using a Newly Screened Aptamer.

ABSTRACT: Owing to the significant roles of adenosine triphosphate (ATP) in diverse biological processes, ATP level is used to research and evaluate the physiological processes of organisms. Aptamer-based biosensors have been widely reported to achieve this purpose, which are superior in their flexible biosensing mechanism, with a high sensitivity and good biocompatibility; however, the aptamers currently used for ATP detection have a poor ability to discriminate ATP from adenosine diphosphate (ADP) and adenosine monophosphate (AMP). Herein, an ATP-specific aptamer was screened and applied to construct a fluorescent aptasensor for ATP by using graphene oxide (GO) and strand displacement amplification (SDA). The fluorescence intensity of the sensor is linearly related to the concentration of ATP with

SUBMITTER: Chen X 

PROVIDER: S-EPMC9003339 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature