Changing practice: red blood cell typing by molecular methods for patients with sickle cell disease.

ABSTRACT:

Background

Extended red blood cell (RBC) antigen matching is recommended to limit alloimmunization in patients with sickle cell disease (SCD). DNA-based testing to predict blood group phenotypes has enhanced availability of antigen-negative donor units and improved typing of transfused patients, but replacement of routine serologic typing for non-ABO antigens with molecular typing for patients has not been reported.

Study designs and methods

This study compared the historical RBC antigen phenotypes obtained by hemagglutination methods with genotype predictions in 494 patients with SCD. For discrepant results, repeat serologic testing was performed and/or investigated by gene sequencing for silent or variant alleles.

Results

Seventy-one typing discrepancies were identi

SUBMITTER: Casas J 

PROVIDER: S-EPMC9003876 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature