Endurance exercise attenuates juvenile irradiation-induced skeletal muscle functional decline and mitochondrial stress.

ABSTRACT:

Background

Radiotherapy is commonly used to treat childhood cancers and can have adverse effects on muscle function, but the underlying mechanisms have yet to be fully elucidated. We hypothesized that endurance exercise following radiation treatment would improve skeletal muscle function.

Methods

We utilized the Small Animal Radiation Research Platform (SARRP) to irradiate juvenile male mice with a clinically relevant fractionated dose of 3× (every other day over 5 days) 8.2 Gy X-ray irradiation locally from the knee to footpad region of the right hindlimb. Mice were then singly housed for 1 month in cages equipped with either locked or free-spinning voluntary running wheels. Ex vivo muscle contractile function, RT-qPCR analyses, resting cytosolic and sarcoplasmic reticulum

SUBMITTER: O'Connor TN 

PROVIDER: S-EPMC9004104 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature