COX-2 strengthens the effects of acid and bile salts on human esophageal cells and Barrett esophageal cells.

ABSTRACT:

Aims

Investigate the effect and mechanism of COX-2 on viability, intestinal metaplasia, and atypia in human esophageal squamous and Barrett esophageal cell lines.

Methods

Human esophageal squamous and Barrett esophageal cell lines were transfected with a COX-2 expression vector and a COX-2 siRNA, and then were treated with acid, bile salts, and a mixture of both. Cell viability, the expression of COX-2, NF-κB(p65), CDX-2, MUC2, c-myb, and BMP-4, and the morphology and microstructure of cells were then observed.

Results

The viability of COX-2 overexpressed cells was significantly higher than that of control cells, while the viability of COX-2 siRNA-treated cells was significantly lower than that of control cells. Intestinal metaplasia and atypia were observed in cells

SUBMITTER: Jiangang S 

PROVIDER: S-EPMC9004192 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature