Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum.

ABSTRACT: The majority of the genes present in bacterial genomes remain poorly characterized, with up to one-third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput technique that can help rectify this deficiency. The technology, however, can only be realistically applied to those species in which high rates of DNA transfer can be achieved. Here, we have developed a number of approaches that overcome this barrier in the autotrophic species Clostridium autoethanogenum by using a mariner-based transposon system. The inherent instability of such systems in the Escherichia coli conjugation donor due to transposition events was counteracted through the incorporation of a conditionally lethal codA marker on the plas

SUBMITTER: Woods C 

PROVIDER: S-EPMC9004356 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature