Laminin matrix regulates beta-cell FGFR5 expression to enhance glucose-stimulated metabolism.

ABSTRACT: We previously showed that pancreatic beta-cells plated on laminin matrix express reduced levels of FGFR1, a receptor linked to beta-cell metabolism and differentiation. Due to recent evidence that adult beta-cells also express FGFR5, a co-receptor for FGFR1, we now aim to determine the effect of laminin on FGFR5 expression and consequent effects on beta-cell metabolism. Using a genetically encoded sensor for NADPH/NADP⁺ redox state (Apollo-NADP⁺), we show overexpression of FGFR5 enhances glucose-stimulated NADPH metabolism in beta-cell lines as well as mouse and human beta-cells. This enhanced response was accompanied by increased insulin secretion as well as increased expression of transcripts for glycolytic enzymes (Glucokinase/GCK, PKM2) and the functional maturity

SUBMITTER: Pal V 

PROVIDER: S-EPMC9005713 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature