Molecular identification of Malassezia species isolated from neonates hospitalized in Neonatal intensive care units and their mothers.

ABSTRACT:

Background and purpose

Given the important role of Malassezia spp. in skin diseases and other associated infections in neonates, this study aimed to investigate the presence and frequency of Malassezia spp. in the skin of neonates hospitalized in neonatal intensive care units and their mothers using culture and accurate molecular-based methods.

Materials and methods

In total, 205 samples were collected from 130 neonates (>4-day-old) and 75 mothers. Isolation of Malassezia spp. from the skin was performed using Leeming-Notman agar and modified Dixon agar media. To compare the Malassezia microflora on the skin of the neonates and their mothers, a polymerase chain reaction-sequencing method was performed for spp. identification of 92 isolates obtaine

SUBMITTER: Zomorodian K 

PROVIDER: S-EPMC9006734 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature